Protein Purification Reagents and Kits
System Biosciences LLC ExoQuick-TC ULTRA for Tissue Culture MediaExoQuick ULTRA EV Isolation Kit for Tissue Culture Media = 20 Reactions
ExoQuick-TC ULTRA is our quickest, cleanest method for isolating EVs from most biofluids. Leveraging our years of exosome expertise, the SBI team has pushed ExoQuick-TC extracellular vesicle (EV) isolation technology to new peaks of performance with ExoQuick-TC ULTRA for tissue culture media and most biofluids. While many EV isolation methods force you to choose between high yields, easy protocols, clean preps, and low costs, ExoQuick-TC ULTRA is able to deliver on all these fronts for trade-off free EV preparation.
IBA LifeSciences GFP-Strep-tag control protein Strep-tagII; 500 µg
GFP-Strep-tagII is a control protein consisting of the green fluorescent protein (GFP) C-terminally tagged with the Strep-tagII. It is suitable for test purifications, detections, and immobilizations with Strep-Tactin or Strep-TactinXT.
IBA LifeSciences 100 mM Biotin stock solution; 1 ml
Biotin stock solution for preparing a dissociation buffer when using the Strep-tag technology for cells (Fab-TACS/Nano-TACS, CD3/CD28 Streptamer, MHC I Streptamer). Biotin addition causes the rapid dissociation of isolation reagents from the cell surface. Thus, fully functional, highly pure, and non-induced cells are received.
IBA LifeSciences Strep-TactinXT 4Flow cartridge; 5 ml
Strep-TactinXT 4Flow cartridges contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins with HPLC/FPLC devices like Äkta systems (Cytiva). Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow allows the application of denaturing agents such as urea.
IBA LifeSciences Strep-tagII peptide; 1.8 mg
Synthetic Strep-tagII peptide recognized by Strep-Tactin, Strep-TactinXT as well as the monoclonal antibodies, StrepMAB-Classic and StrepMAB-Immo. It can be used for competitive elution of Strep-tagII fusion proteins or as control protein in dot blot assays.
IBA LifeSciences Strep-TactinXT 4Flow cartridge; 1 ml
Strep-TactinXT 4Flow cartridges contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins with HPLC/FPLC devices like Äkta systems (Cytiva). Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow allows the application of denaturing agents such as urea.
IBA LifeSciences Strep-TactinXT 4Flow Starter Kit
Strep-TactinXT 4Flow Starter Kit contains all essential reagents required for purification and detection of Strep-tagII and Twin-Strep-tag fusion proteins. It provides a Strep-TactinXT 4Flow column, Buffer W, Buffer BXT, and Buffer XT-R for purification as well as Strep-Tactin HRP for subsequent western blot detection. Strep-TactinXT 4Flow columns contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow allows the application of denaturing agents such as urea.
ACROBiosystems Human iPSC-Derived Cardiac Organoid Maintenance Kit
Human iPSC-Derived Cardiac Organoid Maintenance Kit is expressed from Human
Revvity Health Sciences Inc High Resolution LabChip GXII, Touch HT
High Resolution LabChip GXII, Touch HT
Hampton Research 10% W/V TRYPTONE 100ML
HR2-835/10% w/v Tryptone - 100 ml. Please note items from this supplier are non-returnable.
STEMCELL Technologies EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit, For processing: 20 mL biofluid processed
The EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD9, CD81, and CD63, and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.
STEMCELL Technologies RosetteSep™ Human Granulocyte Depletion Cocktail, For processing: 40 mL whole blood processed
The RosetteSep™ Human Granulocyte Depletion Cocktail is designed to deplete granulocytes from whole blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The granulocyte-depleted fraction is present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Human Progenitor Enrichment with Platelet Depletion, For processing: 1 x 10^9 cells
The EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion is designed to isolate hematopoietic progenitor cells from fresh or previously frozen cord blood and other cell preparations that contain large numbers of platelets by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.